How is a FEC carried out ?
La coproscopie consiste à observer et compter les œufs de parasites excrétés par un cheval dans ses crottins, au microscope. Elle permet de décider de vermifuger ou non un cheval. On observe principalement des œufs de strongles, ascaris et strongyloïdes. Après un prélèvement de crottins frais, celui-ci peut être conservé au réfrigérateur en attendant de réaliser l'analyse. Plusieurs méthodes de coproscopies existent. Seule la méthode quantitative de Mac Master, classiquement utilisée, est présentée ici.
- Equipment needed for carrying aout a FEC
- MacMaster quantitative approach
- Interpreting the Mac Master slide
- Recognising the different parasites
- Interpretation of the results
Equipment needed for carrying aout a FEC
For collection of a sample of droppings : Sugical glove
To identify the sample : a permanent marker pen, for preservation (in a refrigerator)
For the analysis :
• Pair of tweezers
• Small bowls
• Accurate electronic weighing scales (accuracy : 0.01g, capacity : 100g)
• Flotation liquid : High density solution, allowing egg concentration, lower density on the surface. E.g saturated salted water (300g of salt per litre of water)
• A measuring glass calibrated in ml, or a 50ml seringe
• A tea strainer
• Plastic pipettes
• A MacMaster slide : special laboratory slide with two chambers. Each chamber has a network where the limits are engraved as a square divided into columns.
• A microscope with a X10 Power lense
MacMaster quantitative approach
Interpreting the Mac Master slide
Interpretation of the Mac Master slide under the microscope is done in the following way :
• Adjust the lens and focus on the lines of the grid, and on the air bubbles
• Reading the slide and counting the eggs of each species of parasite, in both grids, following th lines etched in the slide
• Calculation : : multiply the number of eggs counted by 50.
Recognising the different parasites
Interpretation of the results
Treatment thresholds vary according to the parasites concerned. A count of 200 epg is commonly used as a threshold for treating against small strongyles. However, it is necessary to have epidemiological knowledge before any decision is made. Consult your treating veterinarian for advice.
Know more about our authors
- Translated from french by : Karen DUFFY Translator
- Marie DELERUE Veterinarian - development engineer IFCE